Assessing the effects of a novel biostimulant to enhance leafminer resistance and plant growth on common bean

Amr A Mostafa,Hanaa S Omar,Soheir N Abd El-Rahman,Naglaa A Abdallah,Said Shehata

doi:10.1038/s41598-021-98902-z

Amr A Mostafa, Hanaa S Omar + Show 3 more

Open Access

https://doi.org/10.1038/s41598-021-98902-z

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Journal: Scientific Reports	Publication Date: Oct 8, 2021
Citations: 4	License type: open-access

Affiliation: Cairo University, Agricultural Research Center

Abstract

The leafminer Liriomyza trifolii is one of the major insects that affect Phaseolus vulgaris production worldwide. Novel and safe biobased stimulator compound (BSTC) with micronutrient-amino acid chelated compounds was developed from natural compounds and was used for foliar spray of P. vulgaris. Treated plants showed significantly increased in quality and productivity as well as significant reduction in leafminer infestation by close the tunnel end resulting in larvae suffocation and death. BSTC contains chemical composition that has important function in inducing immunity and resistance against insects, enhance plant growth and production. Also, HPLC showed that the assembled BSTC is rich in nucleobases than yeast extract (> 56 fold). Aminochelation zinc enhanced the rate of absorption of nutrient compounds and could participate in safe biofortification strategy. The expression of plant defense related genes under BSTC treatment revealed strong correlations between the transcription rates of defense related genes. Based on binding energies and interacting residues of six vital insect proteins, the best-docked complexes was obtained with disodium 5′-inosinate, delphinidin 3-glucoside and hyperoside. Obtained findings indicate that the foliar application of BSTC can enhance plant growth and productivity, uptake of important elements, expression of defense related genes and inhibit insect essential genes.

Highlights

The leafminer Liriomyza trifolii is one of the major insects that affect Phaseolus vulgaris production worldwide
We investigated the presence of important chemical compounds using HPLC and gas chromatography-mass spectrometry (GC–MS) in the developed bioactive stimulator and explored their influence on gene expression of plant defense related genes, and listed the most binding molecules to vital insect proteins by molecular docking approach to understand the biological activities involved in gene inactivation
Inexpensive, safe and prepared biobased stimulator compound were formulated with higher nutrient values was developed to avoid the use of any toxic auxiliary substances

Summary

Introduction

The leafminer Liriomyza trifolii is one of the major insects that affect Phaseolus vulgaris production worldwide. Obtained findings indicate that the foliar application of BSTC can enhance plant growth and productivity, uptake of important elements, expression of defense related genes and inhibit insect essential genes. The larvae feed and damage the foliage, while the female adults puncture plant tissue during oviposition, both stages reduce photosynthesis, cause defoliation and result in yield loss, while damage crop plants have a role in leaf damage as larvae primarily mine the palisade mesophyll, feed and damage the foliage[3,4] They are difficult to control as they are protected inside the mines, synthetic insecticides are the main strategy in the management of insect pests, these chemical substances increase insect resistance over successive generations, negative effects on non-target organisms and are threat to human health. Antioxidants and plant growth promoting compounds such as nitrogen components, salicylic acid (SA), ascorbic acid, etc. have been used as stimulators for increasing the yield and quality of crops and fruits[20,21]

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