Assessing the effect of developed ayurvedic food for ADHD children on animal models

Abstract

As stated by World Health Organization (WHO), a cerebral disarray are to amplify by 50 per cent in the year of 2020, is the fore most finding root of morbidity in kids. The kids represent on 40 per cent in India and the rate of psychopathology amid childhood is 5 to 15 per cent (The Global Economic burden of non-communicable diseases, 2011). ADHD is a multi factorial, highly heritable disorder, estimating a mean heritability of 76 percent (Farone and Mick, 2010). The spontaneously hypertensive rat (SHR) has been used as a genetic animal model of attention deficit/hyperactivity disorder (ADHD). The open field test for loco motor activity, Y-maze test for inattention and Electro-foot shock aversive water drinking test (EFSD) for impulsivity were analyzed using one-way analysis of variance (ANOVA). However, impulsive behavior in the Ymaze tests, which is designed to detect anxiety-related behavior but also reflects impulsivity for novelty seeking, was comparable to Hyperactivity and inattention. ADHD like behaviors in SHR were ameliorated with methylphenidate at a low dose (0.05 mg/kg, i.p.). Rats were acclimated to their home cages for at least 7days before testing and all the rats were provided with commercially available normal diet. The animals were given fresh ayurvedic based health mix diet with 3 per cent, 6 per cent and 12 per cent incorporation of medhya rasayana (Centella asiatica and Bacopa monneri) daily for 100 g for each rat and leftover food was collected on next day measured and discarded. The body weights of each rat were measured every week. All the protocol and experiments were conducted in compliance with the ethical principles and guidelines provided by the committee. The experimental groups, consisting of 6 animals per drug and dose, were chosen by means of a randomized schedule and animal study has been carried out for three months. All tests took place between 9:00 am and 6.00 pm. Animal treatment and maintenance were carried out in accordance with the Principles of Laboratory Animal Care. Key words: ADHD, Bacopa monnieri, Centella asiatica, Medya rasayana, SHR.