Assessing the Carboxymethylcellulose Copper-Montmorillonite Nanocomposite for Controlling the Infection of Erwinia carotovora in Potato (Solanum tuberosum L.)

Ryan Rienzie,Marian Brestic,Pavla Vachova,Lasantha Sendanayake,Akbar Hossain,Devika De Costa,Nadeesh M Adassooriya,Milan Skalicky

doi:10.3390/nano11030802

Abstract

A novel antimicrobial formulation based on carboxymethylcellulose (CMC) spray-coated Cu2+ intercalated montmorillonite (MMT) nanocomposite material was prepared and its morphology, internal structure, and bonding interactions were studied. Meanwhile, the antibacterial efficacy and release behavior of Cu2+ was also determined. PXRD patterns indicated the intercalation of Cu2+, while FTIR spectra and TGA traces confirmed the association of Cu−MMT with CMC. SEM study revealed the improvement of nanocomposites by CMC, without disturbing the clay structure. TEM and EDAX studies indicated the distribution of Cu (copper) throughout the composite. In vitro antibacterial assays performed with Erwinia carotovora revealed effective bacterial growth suppression, indicating the potential of this material in controlling soft rot of potatoes (Solanum tuberosum); also observed was a connection between growth inhibition and concentration of CMC spray coats indicating a positive relationship between Cu2+ release and concentration of the CMC coatings. The activity pattern of the nanocomposite displayed a significant degree of sustained-release behavior.

Highlights

Nanoscience-based products and applications have fetched a greater attention in a range of fields including the biocide industry
Inorganic Cu compounds with fungicidal and bactericidal properties, and as fertilizer additives are very popular due to their low cost [3]. Another advantage possessed by copper is that bacteria and fungi cannot build up resistance against it as they do with antibiotics and synthetic fungicides that are organic in origin
The results clearly indicate that the diameter of the inhibition fects, the specifical concentration of spray coat and weight were highly significant correzone increased with the number of composites tested, indicating the antibacterial activity spondingly with p = 0.006 and p = 0.000 values (p < 0.05) to the diameter of the inhibition of all types of nanocomposites used in the study against E. carotovora

Summary

Introduction

Nanoscience-based products and applications have fetched a greater attention in a range of fields including the biocide industry. The use of Cu in agricultural applications goes back to the 19th century. Inorganic Cu compounds with fungicidal and bactericidal properties, and as fertilizer additives are very popular due to their low cost [3]. Another advantage possessed by copper is that bacteria and fungi cannot build up resistance against it as they do with antibiotics and synthetic fungicides that are organic in origin. Among the Cu based biocides, Cu(NO3 ) , CuSO4 .5H2 O, Cu(OCl) , Cu(OH) , and Cu2 O are more common, with the metallic copper

Methods

Discussion

Conclusion