Assessing the antibiofilm activity of flavonol esters against Pseudomonas aeruginosa PAO1 biofilm: an in vitro, molecular docking, and molecular dynamics study

Abstract

Pseudomonas aeruginosa is one of the opportunistic pathogens that may cause serious health problems and can produce several virulence factors, which are responsible for various infections, particularly in immunocompromised patients. They are responsible for producing infections on indwelling medical devices by attaching on to them and forming a biofilm. Antibiofilm, antivirulence, and gene expression studies of P. aeruginosa biofilm treated with esters of flavonols were evaluated. Pyocyanin, cell surface hydrophobicity, LasA protease estimation, rhamnolipid estimation, and pyoverdine estimation were performed to evaluate the antivirulence activities of the test compounds against P. aeruginosa. Previous studies on the antivirulence activity of flavonoids against P. aeruginosa demonstrate that even if they can inhibit bacterial growth, relatively high concentrations of the compound are generally required for the inhibition of virulence factors. The esters showed more than 40% inhibition in all the tested virulence factors at their sub minimum inhibitory concentration. The gene expression studies of selected esters toward lasB and rhlA genes show downregulation of rhlA which suggests the inhibition in biofilm formation through rhamnolipid inhibition, quorum sensing inhibition, or biofilm formation inhibition. Communicated by Ramaswamy H. Sarma