Abstract
three concentrations (250, 500, 1000) µg/ml of sage Salvia officinalis L. leaf aqueous extract were assessed for their anti-mutagenic potentials in cultured blood cells of 10 acute lymphocytic leukaemia (ALL) patients and a similar number of apparently healthy controls. The parameter of assessment was micronucleus (MN) formation, which was either spontaneous or induced by a treatment with the mutagen cytostar 125 µg/ml, and accordingly, eight cultures were set-up. Blood cells in culture I was negative control (untreated cells), while in culture II, the cells were treated with the mutagen cytostar (cytarabine) at a concentration 125 µg/ml. Cultures III, IV and V were treated with the three concentrations of sage extract respectively, while in cultures VI, VII, VIII, interactions between the extract (the three concentrations respectively) and cytosar were carried out. The results demonstrated that ALL patients showed a significant increased frequency of MN formation in the eight cultures as compared to the corresponding cultures in healthy controls. With respect to the spontaneous formation of MN formation (cultures III, IV, V), the three concentrations of sage extract demonstrated a gradual significant reduction of MN frequency in patients (0.0144, 0.0098, 0.0062) MN/cell, respectively and control subjects (0.0104, 0.0076, 0.0038) MN/cell, respectively. In the induction cultures (VI, VII, VIII), a similar reduction was observed and the dose 1000 µg/ml exerted the highest reduction in both patients and controls (0.0108, 0.0086) MN/cell, respectively. These results demonstrate the anti-mutagenic importance of sage leaf aqueous extract.
Highlights
المیرمیة في خلایا المزارع الدمیة لعشرة من مرضى ابیضاض الدم اللمفي الحاد وعدد مماثل من أفراد
These results demonstrate the anti-mutagenic importance of sage leaf aqueous extract
The present investigation demonstrated that the sage leaf aqueous extract was significantly effective in reducing the spontaneous or cytosar-induced formation of micronuclei in healthy subjects or all patients; it is possible to suggest that such extract can be considered as anti-mutagenic agent with the potential to restore the integrity of genetic make-up
Summary
المیرمیة في خلایا المزارع الدمیة لعشرة من مرضى ابیضاض الدم اللمفي الحاد وعدد مماثل من أفراد. These results demonstrate the anti-mutagenic importance of sage leaf aqueous extract. بأفراد السیطرة وللمزارع الخلویة الثمانیة فكان للتركیز الثلاث لمستخلص المیرمیة قابلیة تدریجیة معنویة في خفض تكرار النوى الصغیرة في،(V 0.0076، 0.0104) ( نواة صغیرة على التوالي وأفراد السیطرة0.0062، 0.0098، 0.0144) المرضى فقد لوحظ نمطا،(VIII، VII، VI) أما في المزارع المستحثة المزارع.
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