Assessing small-scale freshwater microplastics pollution, land-use, source-to-sink conduits, and pollution risks: Perspectives from Japanese rivers polluted with microplastics

Abstract

Rivers are vital for understanding freshwater microplastics pollution, along with the conduits from land-sources to marine-sinks. In this study, we investigated microplastics in the small-scale Awano and Ayaragi rivers, which flow into the Sea of Japan (SJ), and the Asa and Majime rivers, which flow into the Seto Inland Sea (SIS) in Yamaguchi Prefecture, Japan. Surface water samples were collected from 29 stations. Filtration, wet peroxidation, and density separation methods were employed to extract microplastics. Polymers were identified via attenuated total reflectance-Fourier transform infrared spectroscopy. Microplastics abundances and comparisons among different rivers revealed that these small-scale rivers were highly polluted than others around the world. Characterization demonstrated that small microplastics (<1000 μm) in size, fibers and fragments in shape and the polymers—polyethylene, polypropylene, vinylon, polyethylene terephthalate, and polystyrene were dominant. These small-scale rivers emitted substantially higher quantities of Japan land-sourced microplastics (0.4–154.27 billions/day and 0.01–17.55 tons/day) into the SJ and SIS environments than larger rivers in other countries compared to basin areas. The pollution load index indicated that all the river stations were polluted with microplastics. An assessment of the polymeric and pollution risks revealed variably low to high risks. The higher were the abundances of microplastics and toxic polymers, the higher were the pollution level and risks. The sites at high risk of pollution were regarded as hotspots. Both point and non-point land-uses sources of pollution could release microplastics into the river freshwater environments, affected posing high risks and hotspots. Moreover, the pollution characteristics (shapes-sizes-polymers) indicated serious ecotoxicological threats to these rivers and their downstream environments. This study provided new insights into river microplastics pollution and revealed small-scale rivers to be prominent source-to-sink microplastics conduits. Risk assessments provided a baseline for future comprehensive assessments and developing practical approaches to wards setting water quality criteria, pollution control and management.