ASSESSING PATHOGENIC MUTATIONS IN DENTAL FOLLICLES

Abstract

Objective: While ameloblastomas show BRAF P.V600 E mutations, adenomatoid odontogenic tumors harbor KRAS P.G12 R/V. Considering the fact that ameloblastoma and adenomatoid odontogenic tumors can originate from dental follicles, we hypothesized that the BRAF and KRAS variations might be early events in odontogenic tumor tumorigenesis. We aimed to assess BRAF and KRAS mutations in dental follicles associated with asymptomatic impacted teeth. Study Design: Forty-eight dental follicles containing odontogenic epithelial remnants were included in the study. As ameloblastomas most often occur in the posterior mandible and adenomatoid odontogenic tumors have a predilection for the anterior jaws, we assessed by allele-specific quantitative polymerase chain reaction (qPCR) the presence of BRAF P.V600 E in 32 dental follicles associated with impacted mandibular third molar teeth and KRAS P.G12 V and KRAS P.G12 R mutations in 16 dental follicle specimens obtained from around impacted anterior teeth. Sanger sequencing was used as an additional method. Results: None of the dental follicle cases tested positive for the mutations. Conclusion: In conclusion, we tried to detect the early genetic events associated with odontogenic tumor development in dental follicles, but we were unable to showcase that BRAF P.V600 E and KRAS P.G12 R or P.G12 V mutations are the early genetic events associated with odontogenic tumor development. Supported by CAPES/CNPq_Brazil Objective: While ameloblastomas show BRAF P.V600 E mutations, adenomatoid odontogenic tumors harbor KRAS P.G12 R/V. Considering the fact that ameloblastoma and adenomatoid odontogenic tumors can originate from dental follicles, we hypothesized that the BRAF and KRAS variations might be early events in odontogenic tumor tumorigenesis. We aimed to assess BRAF and KRAS mutations in dental follicles associated with asymptomatic impacted teeth. Study Design: Forty-eight dental follicles containing odontogenic epithelial remnants were included in the study. As ameloblastomas most often occur in the posterior mandible and adenomatoid odontogenic tumors have a predilection for the anterior jaws, we assessed by allele-specific quantitative polymerase chain reaction (qPCR) the presence of BRAF P.V600 E in 32 dental follicles associated with impacted mandibular third molar teeth and KRAS P.G12 V and KRAS P.G12 R mutations in 16 dental follicle specimens obtained from around impacted anterior teeth. Sanger sequencing was used as an additional method. Results: None of the dental follicle cases tested positive for the mutations. Conclusion: In conclusion, we tried to detect the early genetic events associated with odontogenic tumor development in dental follicles, but we were unable to showcase that BRAF P.V600 E and KRAS P.G12 R or P.G12 V mutations are the early genetic events associated with odontogenic tumor development. Supported by CAPES/CNPq_Brazil