Assessing Myo‐mechanicals Characteristic of Rectus Abdominis Muscle after Stem Cell Therapy

Abstract

Hyperglycemia during gestation have the deleterious effect in the skeletal muscle leading to occurrence of urinary incontinence and pelvic floor muscle dysfunctions in women. Induces functional, metabolic and structural changes in skeletal muscle; decreasing the functional capacity of the muscle, currently there is not therapies in diabetic gestational myopathy and the new proposal of muscle regeneration is based in stem cell therapy in biodevice use. To evaluate the functionality of the muscle in the new therapies to investigate the effectiveness of the muscle regeneration by assessing myo‐mechanicals characteristic of rectus abdominis muscle after therapeutic application of biodevice with mesenchymal stem cells in experimental model in diabetic and non‐diabetic pregnant rats. 20 postpartum rats of the Sprague Dawley® specific pathogen free strain, composing IV groups as follows: I. Diabetic group without biodevice (GD), II. Diabetic group with biodevice (GD ‐Bio), III. Non‐diabetic group without biodevice (GND) and IV. Non‐diabetic group with biodevice (GND‐Bio). The study was approved by the Botucatu Medical School Ethics Committee on the Use of Animals (CEUA) No. 1234/2017. After the euthanasia of the animals, the rectus abdominis samples will be removed and immediately mounted on the supports inside the isolated organ chambers of the Myograph equipment (Model 820MS; Danish Myo Technology®), the segments will be attached and connected to isometric transducers, submerged in solution physiological composition of Krebs [(mM): NaCl, 130; NaHCO3, 14.9; dextrose, 5.5; KCl, 4.7; KH2PO4, 1.18; MgSO47H2O, 1.17 e CaCl22H2O, 1.6]. Maintained at 37 ° C and constantly bubbled with 95% O2 / 5% CO2 mixture being fixed between a force transducer and a fixed unit. Application of stabilization protocol and then electrical stimulation. The parcial results show that the treated group has an increase of 3mN more than the untreated group; this indicates a better myo‐mechanicals condition in non‐diabetic rats. The Biodevice treatment is effective for the development of muscle regeneration therapy, being able to increase the contractile capacity of the rectus abdominis muscle in the model of diabetic and non‐diabetic pregnant rats.Support or Funding InformationThis project is funded for Sao Paulo Research Foundation, FAPESP (number grant 2016/01743‐5)This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.