Abstract
The aim of this study was to monitor changes in leaf spectral reflectance due to phytoaccumulation of trace elements (Cd, Pb, and As) in sunflower mutant (M5 mutant line 38/R4-R6/15-35-190-04-M5) grown in spiked and in situ metal-contaminated potted soils. Reflectance spectra (350–2500 nm) of leaves were collected using portable ASD spectroradiometer, and respective leaves sample were analyzed for total metal contents. The spectral changes were quite noticeable and showed increased visible and decreased NIR reflectance for sunflower grown in soil spiked with 900 mg As kg−1, and in in situ metal-contaminated soils. These changes also involved a blue-shift feature of red-edge position in the first derivatives spectra, studied vegetation indices and continuum removed absorption features at 495, 680, 970, 1165, 1435, 1780, and 1925 nm wavelength. Correlograms of leaf-metal concentration and reflectance values show highest degrees of overall correlation for visible, near-infrared, and water-sensitive wavelengths. Partial least square and multiple linear regression statistical models (cross-validated), respectively, based on Savitzky–Golay filter first-order derivative spectra and combination of spectral feature such as vegetation indices and band depths yielded good prediction of leaf-metal concentrations.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
