Abstract
Two molecular marker systems, random amplified polymorphic DNA (RAPD) and microsatellites, were used to evaluate population diversity and differentiation in 4 northern Nevada Bromus tectorum populations. From 80 RAPD primers, we found 16 (20%) that yielded 165 strong repeatable bands. Of those bands, 60 (35.8%) were polymor- phic. Of those, 21 met data-pruning guidelines for final analysis. RAPD variation was moderate (x = 0.363, s x -= 0.022), ranging from 0.312 to 0.404. Microsatellite variation was similar ( x - = 0.234, s x 0.051) but varied more widely, rang- ing from 0.009 to 0.551. All populations were out of Hardy-Weinberg equilibrium, as expected in a predominantly self- ing species. RAPDs revealed significant differentiation (P < 0.0001) across populations, whereas microsatellites only resolved 2 of the 4 populations. RAPDs revealed a considerable amount of variation in the 2 populations, Hot Springs and Truckee, which had nearly identical microsatellite profiles. Of 184 individuals, we found 182 unique RAPD pheno- types. We found 51 microsatellite genotypes across individuals, one of which was present in 95.3% of the individuals in the Hot Springs-Truckee group and 29% of the Stillwater individuals. The UPGMA phenograms were similar in group- ing Hot Springs with Truckee, and Stillwater with Peavine, and were highly correlated but not significantly. Both marker systems proved useful in assessing population genetic variation. One population-specific RAPD marker and 6 new microsatellite-length polymorphisms were identified. Both marker systems may also prove useful in studying other closely related species such as red brome (Bromus rubens).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.