Assessing Behavioral Impairment after Doxorubicin Chemotherapy in Rats

Abstract

Chemotherapy has been associated with deficits in cognition and memory, delayed processing speed and executive function which may remain in patients as a long-term neurological effects. In addition, it also causes the disruption of hippocampal cell proliferation and neurogenesis, increased oxidative stress, and disruption in white matter. The cellular mechanisms linked with these side effects are poorly understood until now. Doxorubicin is Anthracycline agent that adversely affects the hippocampal neurogenesis and induces apoptosis by increasing oxygen radicals. It is considered first line therapy treatment for the most common cancers like breast, ovarian, and bladder. Young adult female Long Evans Hooded rats were divided into Doxorubicin treated (6mg/kg IP injection; once weekly for 4 weeks) and control groups (IP injection of sterile 0.9% saline on same schedule). We utilized the string pulling task, skilled ladder rung walking task, and other standard cognitive tests. We also performed immunohistochemistry to examine markers of inflammation related to chemotherapy and the extent to which chemotherapy would affect the process of neurogenesis. The results of the experiment we performed will be presented at the meeting and represent preliminary data for future studies.