Assessing δ13C, δ15N and Total Mercury Measures in Epidermal Biopsies From Gray Whales

Marianne Lian,Brooke Griff,Debra Miller,Jennifer Dupont,Vladimir V Vertyankin,Koen Broker,Todd M O’Hara,Céline A J Godard-Codding,J Margaret Castellini

doi:10.3389/fmars.2020.00133

Abstract

The gray whale (Eschrichtius robustus) is recognized as two geographically isolated and somewhat genetically distinct populations, with recent evidence showing exchange that questions the degree of isolation. The western gray whale subpopulation is currently listed as critically endangered, as designated by the IUCN, whereas the eastern North Pacific population is listed by the IUCN as “Least Concern”. The International Whaling Commission has stressed the value of studies on feeding ecology and contaminants for the western subpopulation. Our objective was to test for differences among epidermal tissue strata as part of an optimization effort to best use and interpret data from small biopsy samples. Fresh to moderately decomposed full thickness samples of epidermis and dermis were collected from stranded eastern gray whales along the California coast, USA (N: 14), and biopsy samples (N: 10) were collected from free-ranging western gray whales. We determined 13C and 15N stable isotope ratios (δ13C, δ15N); and total Hg concentrations ([THg]) from histologically different strata of epidermal tissue samples from the eastern gray whale population The information from these assessments (stranded whale samples) were directly applied for determining δ13C and δ15N from biopsies collected remotely from free-ranging western gray whales, as well as evaluating [THg]. We found a significant difference in [THg] values between the different strata. This has implications for analyzing Hg, and possibly other elements, from an epidermal biopsy from a free-ranging gray whale, and we recommend identifying which stratum/strata the biopsy represents. There were no significant differences in δ13C and δ15N values between the different strata, making epidermal biopsies from free-ranging gray whales an ideal sampling method to investigate δ13C and δ15N ratios regardless of strata representation.

Highlights

Total mercury concentration was measured in four different epidermal depths for the eastern gray whale samples (Figure 2)
For samples that were not lipid extracted there was no significant difference in δ15N (F3,42 = 1.84, p = 0.15) or δ13C (F3,42 = 1.77, p = 0.17) values measured from the different epidermal layers for the eastern gray whale samples
We found no differences in δ15N or δ13C values as measured in D3 (F1,8 = 1.24, p = 0.29 and F1,8 = 0.86, p = 0.39, respectively) when comparing eastern and western gray whale populations

Summary

Introduction

Gray whales (Eschrichtius robustus) inhabit shallow coastal waters in the eastern and western coastlines of the North Pacific and are recognized as two geographically isolated and somewhat genetically distinct populations (Bradford et al, 2008; Bickham et al, 2013; Brüniche-Olsen et al, 2018a), with recent evidence showing some exchange (Weller et al, 2012; Lang et al, 2014; Mate et al, 2015; Brüniche-Olsen et al, 2018a). The relative longevity of gray whales sets the stage for bioaccumulation, but gray whales primarily feed on lower trophic levels of benthic invertebrates (Dehn et al, 2006a,b; HorstmannDehn et al, 2012) as compared to piscivorous cetaceans well known to have much higher Hg tissue concentrations. There is no documentation of Hg concentrations sampled from living free-ranging gray whales, possibly biasing the reported Hg concentrations for this species. Hg analysis in different epidermal strata was previously done in beluga (Delphinapterus leucas) and narwhal (Monodon monoceros) (Wagemann and Kozlowska, 2005), but to our knowledge it has not been previously analyzed in gray whales

Objectives

Methods

Results

Conclusion