Abstract
Based on the layer-by-layer self-assembly of positively charged cetyltrimethylammonium bromide (CTAB) wrapped gold nanorods (AuNRs) and negatively charged superoxide dismutase (SOD) from their aqueous solutions on cysteine modified gold electrode (Cys/Au), a third generation electrochemical biosensor ((SOD/AuNRs)2/Cys/Au) for superoxide anion (O2·−) was developed. The two layers assembly of SOD/AuNRs can significantly enhance the direct electron transfer between SOD and the electrode. The functional enzymatic activities of the SOD offer an electrochemical approach to the determination of O2·−. In the reductive regions, the proposed sensor exhibits excellent analytical performances, such as wide linear range (200 nM to 0.2 mM O2·−), low detection limit (100 nM O2·−), high sensitivity (22.11 nA cm−2 μM−1), short response time (less than 5 s), good stability and reproducibility, while no obvious interferences are caused by commonly met interfering species including hydrogen peroxide (H2O2), uric acid (UA) and ascorbic acid (AA).
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