Abstract
The micromanipulation of objects into 3-dimensional geometries within holographic optical tweezers is carried out using modified Gerchberg-Saxton (GS) and direct binary search (DBS) algorithms to produce the hologram designs. The algorithms calculate sequences of phase holograms, which are implemented using a spatial light modulator, to reconfigure the geometries of optical traps in many planes simultaneously. The GS algorithm is able to calculate holograms quickly from the initial, intermediate and final trap positions. In contrast, the DBS algorithm is slower and therefore used to pre-calculate the holograms, which are then displayed in sequence. Assembly of objects in a variety of 3-D configurations is semi-automated, once the traps in their initial positions are loaded.
Highlights
Optical tweezers are used for manipulating both single and multiple, micron-sized, particles suspended in solution [1]
The micromanipulation of objects into 3-dimensional geometries within holographic optical tweezers is carried out using modified Gerchberg-Saxton (GS) and direct binary search (DBS) algorithms to produce the hologram designs
The DBS algorithm is slower and used to pre-calculate the holograms, which are displayed in sequence
Summary
Optical tweezers are used for manipulating both single and multiple, micron-sized, particles suspended in solution [1]. Various types of particles can be trapped including transparent silica or polymer spheres, metallic particles and biological specimens [2]. Recent advances in spatial light modulators (SLMs) enable a single laser beam to be split into many beams, enabling the simultaneous trapping of many objects [3]. Such experimental arrangements are termed holographic optical tweezers (HOTs) [4]. The spatial resolution of the SLM, and the aberrations within the system, limit the maximum displacements to a few 10’s μm
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