Abstract
Plant mitochondrial (mt) genomes possess several complex features, including a variable size, a dynamic genome structure, and complicated patterns of gene loss and gain throughout evolutionary history. Studies of plant mt genomes can, therefore, provide unique insights into organelle evolution. We assembled the complete Salix purpurea L. mt genome by screening genomic sequence reads generated by a Roche-454 pyrosequencing platform. The pseudo-molecule obtained has a typical circular structure 598,970 bp long, with an overall GC content of 55.06%. The S. purpurea mt genome contains 52 genes: 31 protein-coding, 18 tRNAs, and three rRNAs. Eighteen tandem repeats and 404 microsatellites are distributed unevenly throughout the S. purpurea mt genome. A phylogenetic tree of 23 representative terrestrial plants strongly supports S. purpurea inclusion in the Malpighiales clade. Our analysis contributes toward understanding the organization and evolution of organelle genomes in Salicaceae species.Electronic supplementary materialThe online version of this article (doi:10.1186/s40064-016-3521-6) contains supplementary material, which is available to authorized users.
Highlights
Mitochondria contribute to energy metabolism and play fundamental roles in plant development, fitness, and reproduction, as well as being associated with the biosynthesis of fatty acids and several active proteins (Mcbride et al 2006; Ryan and Hoogenraad 2007)
All 52 genes are single copy, with the exception of one transfer RNA (tRNA) gene, which has a duplicated copy, and one tRNA gene, which occurs in triplicate
Protein-coding genes range in length from 2004 bp to 225 bp
Summary
Mitochondria contribute to energy metabolism and play fundamental roles in plant development, fitness, and reproduction, as well as being associated with the biosynthesis of fatty acids and several active proteins (Mcbride et al 2006; Ryan and Hoogenraad 2007). Salix purpurea L. is a willow species native to much of Europe (north to the British Isles, Poland, and the Baltic States), western Asia, and North Africa (Argus 1997; Skvortsov 1999; Sulima et al 2009). It is a deciduous shrub growing 1–3 m tall, with purple-brown to yellow–brown shoots, green foliage, and small purple or red catkins produced in the early spring. Purple willow bark contains a valuable raw material traditionally used for the production of natural aspirin and other salicylic glycosides with
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