Abstract
Glucose tolerance factor (GTF) has usually been assayed by manometric measurement of CO 2 evolved when glucose was metabolizing glucose. By using 14C labeled substrates it has been shown that GTF increases the decarboxylation of pyruvate to ethanol and CO 2. Thus in addition to measuring CO 2 evolution, the enzymatic estimation of the increased ethanol production can be used to assay GTF. A further effect of GTF was to cause increased carboxylation of pyruvate to substrates that are used in the biosynthesis of cell substance. The metabolic sites of action of GTF are discussed.
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