Year-end Sale % OFF 
Abstract
Publisher Summary Positions of nucleosomes are defined translationally and rotationally. Translational positioning specifies where the histone core begins and ends along a DNA sequence. Rotational positioning refers to the orientation of a DNA helix relative to the histone core. Several techniques have been developed to map both the translational and rotational positioning of nucleosomes. These techniques utilize enzymes or chemicals that cleave nucleosomal and nonnucleosomal DNA differentially. Differential cleavage is largely because of the wrapping of DNA around the nucleosome core, which makes DNA in chromatin less accessible to cutting. In many cases, the mechanism of positioning is unclear. Experimental evidence indicates that nucleosome positioning can be directed by a DNA sequence and by DNA-binding proteins that position nucleosomes or generate nucleosome-free regions that influence positioning in adjacent sequences. This chapter discusses the methodologies that are used to assess nucleosome positioning in yeast.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
