Abstract
Regulation of retinal phosphodiesterase (PDE) by the transducins is well known, and essential to signal transduction in both rod and cone photoreceptor cells of the retina. Data suggest that G proteins may regulate other PDE isoforms in nonretinal sensory systems and nonsensory cells. PDEs have been isolated from retinal, gustatory, and pineal tissues and tested them for interactions with multiple G protein α subunits using recombinant G protein subunits, as well as wild-type and substituted peptides corresponding to the so-called effector interaction region of the G protein α subunit. This chapter describes methods for preparing PDEs from sensory tissues and monitoring G protein-PDE interactions using peptides and recombinant proteins.
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