Abstract
A method for determining urease activity in soil columns is described. It is based on a systematic study of factors affecting the release of ammonium when soil columns are perfused with a buffered urea solution (containing KCl to release NH 4 +) under steady-state conditions at a low flow velocity. Effluent emerging from the column is quickly mixed with a urease inhibitor (phenylmercuric borate) and the ammonium produced is determined by steam distillation. The reproducibility of the method is reported. In addition, kinetic constants for urease (calculated from the integrated form of the Michaelis-Menten equation) in column and batch (static and shaken assays) type reactions are compared.
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