Assay of teichoic acid antibodies and antistaphylolysin in the diagnosis of Staphylococcal osteomyelitis.

Abstract

Teichoic acid antibodies (TAA), measured by gel diffusion, and antistaphylolysin (ASa) values were determined in 46 osteomyelitis patients and 200 healthy controls. A positive TAA titer (greater than or equal to 4) was seen in 4/16 patients with acute hematogenous osteomyelitis caused by Staphylococcus aureus and in 7/22 patients with chronic staphylococcal osteomyelitis, while ASta was positive (greater than or equal to 2.0 IU/ml) in 9 patients in both groups all except 2 exceeding 4.0 IU/ml. No positive TAA titres or ASta values were seen in 8 patients with chronic nonstaphylococcal osteomyelitis. The rate of positive test results in 200 controls was 7% for TAA and 4% for ASta. Basing diagnosis on a positive value in either of the tests gave 21 positive results in 38 patients with staphylococcal osteomyelitis; 18 by ASta alone and 11 by TAA alone. Both tests were positive at the same time in 3 patients with acute and in 5 patients with chronic staphylococcal osteomyelitis but never in controls.