Abstract
A method is described for the assay of protocollagen lysyl hydroxylase activity in human skin specimens of 100 to 300 mg wet weight. With the procedure reported here, about 90% of the enzyme activity could be extracted from the skin, and results for six skin samples analyzed separately from the same human subject agreed within a variation coefficient of 13%. The lysyl hydroxylase in human skin resembled that from chick embryos in requiring ascorbate, α-ketoglutarate, ferrous iron and atmospheric oxygen. In human skin the activity was highest in foetuses, and higher in infants than in adult subjects. The mean value in young foetuses was about 40 times, and the mean value in two infants of 2 and 4 months of age about 8 times that found in adult subjects.
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