Abstract
A method is presented for the quantitative determination of progesterone in oil solutions. The ketosteroid is isolated by partition chromatography using nitro-methane on purified siliceous earth as the immobile phase and n-heptane as the eluent. The assay is based on the ultraviolet absorbance in ethanol solution at the maximum near 241 m/i. The identity of the separated progesterone is confirmed by determining the infrared spectrum in carbon disulfide solution. A method is presented for the quantitative determination of progesterone in oil solutions. The ketosteroid is isolated by partition chromatography using nitro-methane on purified siliceous earth as the immobile phase and n-heptane as the eluent. The assay is based on the ultraviolet absorbance in ethanol solution at the maximum near 241 m/i. The identity of the separated progesterone is confirmed by determining the infrared spectrum in carbon disulfide solution.
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