Abstract
A study was undertaken to measure Aroclor 1254 body burdens in two benthic marine copepods, Amphiascus tenuiremis and Microarthridion littorale. A microextraction technique was developed which utilizes 100 μL of total extraction volume and as few as 20 copepods (approximately 25 μg of dry tissue mass). Copepods were homogenized and extracted in a 100-μL closed vial with alkaline media (4 M NaOH, 50 μL) and isooctane (50 μL) spiked with an internal standard (hexachlorobenzene), creating a colloidal suspension. After centrifugation, Aroclor 1254 quantification by GC-ECD was directly determined from the organic solvent phase
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