Abstract
AbstractA novel and simple spectrophotometric method for the determination of phenformin hydrochloride using sodium nitroprusside as chromogenic reagent is developed in this paper. The experiment indicates that the sodium nitroprusside can react with the phenformin hydrochloride in a basic solution to form a product with colored N‐nitrosamines. The stoichiometric ratio of phenformin hydrochloride and sodium nitroprusside is 1:3 in the product. The maximal absorption wavelength (λmax) of the product is 520 nm, ϵ520 = 2.2 × 103 Lmol−1cm−1. A Good linear relationship is obtained between the absorbance (A) and the concentration (C) of phenformin hydrochloride in a wide range of 0.20‐400 μg mL−1. The equation of the linear regression is A = 0.03461 + 0.00907C (μg mL−1) with a linear correlation coefficient of 0.9991. The detection limit is 0.13 μg mL−1, and the relative standard deviation (R.S.D.) is 0.19%. The method is successfully applied to the determination of phenformin hydrochloride in tablet forms, and satisfactory recoveries are obtained.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
