Abstract

A method for the assay of hepatic glucuronyl transferase activity using [ 14C]bilirubin as substrate has been developed. Under the conditions of the assay, the conjugation products from normal rats and guinea pigs have been found to react with diazo reagent to form azo-pigment B. Evidence is presented that liver from congenitally jaundiced (Gunn) rats can metabolize small quantities of bilirubin to diazo-negative metabolites that were not characterized. This method is applicable to the quantities of enzyme derived from the small amounts of liver such as might be obtained in human liver biopsy samples.

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