Abstract
Estradiol receptors (ER) were measured in human breast carcinomas using the gamma emitting ligand 16α[ 125I]iodoestradiol. Competition experiments with excess unlabeled steroids showed that the iodoestradiol was bound specifically by the ER. The K d of the iodo and tritium-labeled estradiol for the receptor were similar with values of 3.4 × 10 −18 M and 2.1 × 10 −10 M respectively. A comparison of ER determination in 25 human breast carcinomas using both [ 3H]estradiol and [ 125I]estradiol gave a correlation coefficient value of 0.97. It is concluded that the use of [ 125I]estradiol should greatly simplify the ER assay and facilitate its introduction into the routine hospital laboratory.
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