Abstract
A GLC procedure for determination of vitamins D2 and D3 is described. By pretreatment consisting of saponification and subsequent esterification of all free hydroxyl groups to their respective propionates, vitamins D2 and D3 can be isolated from the interference usually encountered from other oil‐soluble vitamins during GLC analysis. The procedure described, including the “internal standard” technique, supplies precise data rapidly, with accuracy comparable to that obtained by the vitamin Dassay method given in USP XVII.
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