Abstract
A simple assay for phosphatidylserine decarboxylase is described. Following incubation of a mitochondrial fraction from Saccharomyces cerevisiae with purified, exogenous phosphatidyl[ 3H]serine, the lipid extract is applied to a small DEAE-cellulose column equilibrated in CHCl 3CH 3OH (1:1). The unreacted substrate, phosphatidyl[ 3H]serine, is quantitatively bound by the ion-exchange column while the product, phosphatidyl-[ 3H]ethanolamine, is eluted by sequential washing with CHCl 3CH 3OH (1:1) and CH 3OH. The organic solvents are evaporated, and the amount of radiolabeled phosphatidyl[ 3H]ethanolamine formed by enzymatic decarboxylation is determined by liquid scintillation spectrometry. The reliability of this assay was established by showing that several enzymatic properties of the yeast enzyme, defined by the new assay, were essentially identical to the properties characterized by a more tedious paper chromatographic assay described previously. Virtually identical rates of enzymatic decarboxylation of phosphatidyl[ 3H]serine were also obtained for mitochondrial fractions from pig brain and rat liver when the activities were compared by the column and paper chromatographic methods.
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