Abstract
Objective To observe the effects of β 3 -adrenergic receptor (β 3 -AR) antagonist on myocardial Uncoupling protein2 (UCP 2 ) expression and energy metabolism in chronic heart failure rats. Methods 7 weight-matched normal adult rats (control group), 18 rats with ISO induced heart failure (ISO group) and 21 rats with ISO induced heart failure but received specific β 3 -AR inhibitor SR59230A (ISO+ SR59230A group) for 6 weeks were included in this research. At the end of the study, Echocardiography was performed; the ratio of left ventricular weight and body weight (LVW/BW) was calculated. The expression of β 3 -AR and UCP 2 mRNA in myocardium were detected by reverse transcription-polymerse chain reaction (RT-PCR), the UCP 2 protein in myocardium were detected by western blot. The myocardial contents of creatine phosphate (PCr) and adenosine triphosphate (ATP) were measured by high performance liquid chromatography (HPLC). Results Compared with control group, the cardic function was significantly reduced and myocardial β 3 -AR mRNA significantly increased, UCP 2 mRNA and protein was also significantly increased in ISO group, this change could be attenuated by the treatment with SR59230A, and the expression of myocardial UCP 2 protein negatively correlated with PCr contents. Conclusions In the chronic stage of heart failure, the expression of UCP 2 increases, which causes myocardial energy shortage, SR59230A improves myocardic energy efficiency and cardiac function by means of suppressing the the expression of UCP 2 .
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
