Asporogenous yeasts and their supposed ascosporogenous states: An electrophoretic comparison of enzymes.

Abstract

The relationship of nine Candida species, five Torulopsis species, and five species and two varieties of Kloeckera to the ascosporogenous yeast species considered to be their teleomorphs was studied by electrophoretic comparison of enzymes. Close relationships were seen between fourteen teleomorphs and their anamorphic counterparts, Candida guilliermondii and Pichia guilliermondii, Candida krusei and Issatchenkia orientalis (Pichia kudriavzevii), Candida lambica and Pichia fermentans, Candida melinii and Hansenula wingei, Candida utilis and Hansenula jadinii, Candida macedoniensis and Kluyveromyces marxianus, Candida pseudotropicalis and Kluyveromyces fragilis, Candida parapsilosis (group 2) and Lodderomyces elongisporus, Torulopsis holmii and Saccharomyces exiguus, Torulopsis colliculosa and Torulaspora delbrueckii (Saccharomyces fermentati), Torulopsis stellata and Torulaspora delbrueckii (Saccharomyces rosei), Kloeckera africana and Hanseniaspora vinea, Kloeckera apiculata and Hanseniaspora uvarum, and Kloeckera japonica and Hanseniaspora valbyensis. There was no apparent relation between the four pairs: Candida valida and Pichia membranaefaciens, Candida parapsilosis (group 1) and Lodderomyces elongisporus, Torulopsis molischiana and Hansenula capculata, and Torulopsis candida and Debaryomyces hansenii and Debaryomyces marama. From these results, this zymographic technique is considered to be a useful tool for the study of relalationships between the different states of a yeast species.