Abstract
Objective: Exposure to lower oxygen causes oxidative stress and promotes angiogenesis. Asphyctic neonates have shown higher cord-blood vascular endothelial growth factor (VEGF). We hypothesized that retina and choroid having a different circulatory regulation (choroid lacks vascular auto-regulation) would acutely stimulate angiogenesis in response to short and severe hypoxemia. Methods: Retina/choroid were obtained from hypoxic (FiO2:8% x 30 min) newborn piglets resuscitated with 21% O2 and controls (ventilated with 21% O2) at 6h after birth. Vegfa mRNA expression was determined by real-time PCR. Protein level of hypoxia inducible factor-1alpha (HIF-1alpha), Vegf, phosphorylated p65 (S539), p65, and Gapdh (housekeeping gene) were studied by western blotting. Results: Vegfa mRNA expression in retina and choroid significantly increased during hypoxia (p< 0.01 vs. control). At protein level, Hif-1α did not change significantly with hypoxia or re-oxygenation. Vegf protein did not increase in retina or choroid with hypoxia or re-oxygenation. P65 was significantly more phosphorylated in response to hypoxia (p< 0.05 vs. control), and restored to baseline after re-oxygenation just in retina. Total levels of p65 increased in choroid after 6h of resuscitation (p< 0.05 vs. control). Figure Conclusions:
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