Abstract

The testes of guinea pigs varying in ages up to 10 weeks and the testes of guinea pigs rendered aspermatogenic by immunization with antigen were assayed for RNase, DNase, cathepsin, acid phosphatase, <i>β</i>-glucuronidase, hyaluronidase, sorbitol dehydrogenase, lactic dehydrogenase and leucine amino peptidase. The principal findings were: (1) RNase, DNase and cathepsin were significantly elevated in the aspermatogenic testes. This finding is related to the lysosomal membrane damage mediated by antibody and is discussed from the point of view of cell damage; (2) hyaluronidase is proposed as a marker for aspermatogenic antigen since it appears when ASA is found and is lost when the antigen is lost; (3) the levels of hyaluronidase and other enzymes in aspermatogenic testes indicate a conversion from the mature to the immature type of testes.

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