ASPERGILLUS TERREUS KMBF1501 A POTENTIAL PIGMENT PRODUCER UNDER SUBMERGED FERMENTATION

P Akilandeswari,B V Pradeep

doi:10.22159/ijpps.2017v9i4.16176

Abstract

Objective: The present study was aimed to identify the fungal isolate from soil and to understand the different optimized parameters better to facilitate the pigment production that has high yield and stability.Methods: Aspergillus sp. was isolated from Western Ghats soil by the conventional serial dilution technique and assessed as a potential pigment producer. Different broth medium such as potato dextrose broth (PDB), czapek-dox broth (CDB), malt extract broth (MEB), rose bengal broth (RBB), sabouraud dextrose broth (SDB), yeast malt extract broth (YEMB), pH (3-9), temperature (24, 27, 30, 33, 37 and 40 °C), carbon (lactose,glucose,sucrose, maltose, galactose and fructose) and nitrogen source (peptone, yeast extract, urea and inorganic nitrogen sources like potassium nitrate, ammonium chloride and sodium nitrate), mineral salts such as sodium dihydrogen phosphate (Na2H2Po4), magnesium sulphate (Mg2So4), calcium chloride (CaCl2), copper sulphate (Cu2So4), potassium dihydrogen phosphate (KH2Po4) and manganese sulphate (Mn2So4) and inoculum age (2-7 d) of the medium related to high pigment production were analysed.Results: Aspergillus terreus KMBF1501 was identified by ribosomal DNA sequencing showing 99% similarity with other Aspergillus terreus and the Accession number (KX113516) was assigned. The optimum culture conditions for pigment production by Aspergillus terreus KMBF1501 was achieved at pH 5 (0.563±0.012 nm), temperature of 27 °C (0.382±0.001 nm) with glucose (0.501±0.002 nm) as carbon source, peptone (2.147±0.004 nm) as nitrogen source, Mg2SO4 (0.401±0.001 nm)as mineral salt and 4 d (0.324±0.001 nm) of inoculum age in PDB (0.761±0.006 nm).Conclusion: Aspergillus terreus KMBF1501 produced maximum pigment when cultured in modified PDB than in common PDB medium. The high concentration of the pigment can be used for various industrial purposes.

Highlights

Diverse group of fungi are known to harbor in soil and the Western Ghats are considered to be the hot spot location for the biodiversity including microorganisms [1]
The isolated colonies were subjected for the pigment production and following to it screening among the soil samples collected, Aspergillus sp. showed the maximum yield by producing a yellow color pigment that had the high diffusing ability in both the solid and liquid medium respectively
Aspergillus terreus KMBF1501 isolated from the Western Ghats soil produced yellow color pigment was regulated by various factors in the culture medium

Summary

Introduction

Diverse group of fungi are known to harbor in soil and the Western Ghats are considered to be the hot spot location for the biodiversity including microorganisms [1]. Fungi produce an inspiring array of secondary metabolites [3] and the benefits range like the beneficial bioactive compounds such as antibiotics, anticancer, antimicrobial and antioxidants. The discoveries of these secondary metabolites have more interest for the pharmaceutical and agrochemical industries [4]. Pigments derived from the natural sources are increased due to the disquiet of the potential toxicity of several synthetic dyes [6] which are used extensively in the food, cosmetics and pharmaceutical industries [7]. The pigment production in submerged culture is affected by several environmental factors It is mostly affected by the nitrogen source and pH of the medium [8]. Considering the toxic effects of the synthetic dyes, the natural dyes are eco-friendly and degradable since they cause no detrimental effects [11]

Methods

Results

Discussion

Conclusion