Abstract
Aspergillus niger (ATCC 6275, A-98) was mutated by gamma rays and N-methyl- N′-nitro- N-nitrosoguanidine. Selected mutants were tested in fermentation on apple distillery waste substrates to get improved hydrolytic activities and technological properties, e.g. improved filtration, reduced values for chemical oxygen demand and higher raw protein content in the dry filter cake. A scheme of the combined mutagenic treatment is given. A mutant selected after the fourth series of mutagenic treatment was compared with the parent strain and found to have four times higher endo-1,4-β- d-glucanase (1,4(1,3;1,4)-β- d-glucan 4-glucanohydrolase, EC 3.2.1.4) and exo-1,4-β- d-glucanase (1,4-β- d-glucan cellobiohydrolase, EC 3.2.1.91) activities, ten times higher aryl-β- d-glucosidase activity and essentially higher pectolytic and amylolytic activities than the parent strain.
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