Abstract
Fungal diseases are frequently occur in fish culture. The aim of this research was to find out the diversity of Aspergillusassociated with fungal diseases in catfish and Tilapia based on 16S rDNA gene sequences in central Java Indonesia. The combination between exploratory in the field and experiment, method were applied. In order to find out the Aspergillus prevalence, 48 fish were collected from fish pond of Demak, Klaten and Semarang Regency. Based on the clinical sign, 24 moribund fish were chosen for fungus isolation. As a result, 21 fungi isolates (FTD01–FTD05; FTK01-FTK08; FCB01-FCB08) were gained from external wounds of fish with Sabouraud Dextrose Agar (SDA) medium. Based on the Postulate Koch result showed that three isolates (FTD03, FTK07 and FCB01) that were caused 20 – 80% of fish get sick and mortal. On the basis of sequence 16S rDNA analysis, the result showed that FTK01, FTK07, and FCB01 were closely related toAspergillus flavus (100%); Aspergillus niger (71%)and Aspergillus fumigatus (77%) respectively.
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