Asparaginase action: Inhibition of protein synthesis in rat liver mitochondria and microsomes and brain mitochondria and inhibition of glycoprotein synthesis in liver and brain mitochondria by asparaginase

Abstract

Asparaginase inhibition of protein and glycoprotein synthesis in isolated mitochondria and microsomes was studied over a 10 5 concentration range. At 33 mg/ml, asparaginase inhibited protein synthesis in rat liver mitochondria 60%, in rat cerebral cortical mitochondria 55%, and in rat liver microsome 53%. At 33 mg/ml of asparaginase glycoprotein synthesis in rat liver mitochondria was inhibited 60% and in rat cerebral cortical mitochondria 40%. At 10 −3 mg/ml of asparaginase no inhibition of any system occurred.