Abstract
The present study was conducted to compare the sensibility of different methods (DAS-ELISA and RT-PCR) which are used to detect the agents of viral diseases in grapevine. Purposive sampling was done in vineyards in Manisa and Izmir provinces and a total of 50 samples were taken from grapevines showing virus symptoms. DAS-ELISA and RT-PCR methods were used for identification of viral agents in these samples. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcription. PCR products were analysed by gel electrophoresis and visualized by ethidium bromide staining. DAS-ELISA test results showed that the plants were infected with GFkV, ArMV, GLRV 1, 2, 3 and 4-9 viruses. PCR analysis confirmed DAS-ELISA results.
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