Asiaticoside promotes heart autonomic nerve repair through receptor activator of nuclear factor kappa-B ligand pathway in coronary heart disease

Abstract

This experiment intends to observe the effects of Asiaticoside on angiotensin II (AngII)-induced coronary heart disease (CHD) by regulating nuclear factor κ-B ligand receptor activator and RANK pathway (RANKL/RANK). Cardiomyocytes of rats with CHD were divided into AngII group (2 μmol/L), blank control group, and asiaticoside group (2 μmol/L and 10 μmol/L). After 24 hours of intervention, proliferation and apoptosis was assessed. At the same time, the expressions of RANKL, RANK and α-smooth muscle actin (α-SMA) were measured. Compared with blank group, the proliferation ability of cardiomyocytes with CHD in AngII group was significantly decreased and it increased after asiaticoside treatment (P <0.05). The apoptosis of cardiomyocytes in AngII group was higher than blank group and asiaticoside group. Cardiomyocytes proliferation in asiaticoside group was the most obvious (P <0.05). α-SMA expression decreased in AngII group and increased after asiaticoside treatment (P <0.05). RANKL and RANK mRNA in CHD were significantly upregulated after administration of asiaticoside (P <0.05). RANKL/RANK expression was high in normal CHD cardiomyocytes and reduced after Ang II stimulation. Asiaticoside promotes cardiomyocyte proliferation in CHD by promoting RANKL/RANK signaling, thereby reducing the possibility of heart failure in CHD.