Asian corn borer pheromone binding protein 3, a candidate for evolving specificity to the 12-tetradecenyl acetate sex pheromone

Abstract

Most moth species in the genus Ostrinia use varying ratios of (Z)-11- and (E)-11-tetradecenyl acetate as their main sex pheromone components. The Asian corn borer is unique within the genus having evolved to use pheromone components with a shift in the location of the double bond, (Z)-12- and (E)-12-tetradecenyl acetate. We identified cDNAs representing five pheromone binding proteins (PBPs) and two sensory neuron membrane protein genes from an antennal transcriptome. The coding regions of the orthologous genes were cloned from the Asian corn borer and the (E) and (Z) sex pheromone races of the European corn borer. Their nucleotide sequences and transcript expression levels were analyzed to identify candidate genes from the Asian corn borer that may have evolved specificity to the 12-tetradecenyl acetate ligand. PBP2 and PBP3 transcripts were expressed at high male-biased levels. PBP3 had the most nonsynonymous nucleotide substitutions resulting in ten amino acid changes. Based on the predicted three-dimensional structure of PBP3, six of these ten amino acid changes occur in domains that may interact with the pheromone ligand. Future studies will determine whether PBP3 has evolved specificity to the Asian corn borer sex pheromone.