Ash, Carbon Isotope Discrimination, and Silicon as Estimators of Transpiration Efficiency in Crested Wheatgrass

Abstract

Breeding and selection for higher transpiration efficiency (W) has been hampered by tedious and costly methodology. Rapid and less costly methods are needed for screening W in plant improvement programmes. We report the relationship of ash, silicon (Si) concentration, and Si uptake to W in crested wheatgrass (Agropyron desertorum Fischer ex Link] Schultes), an important C3 range grass in western North America. Clones of crested wheatgrass were grown under three water levels in a field rainout shelter and as potted plants under two water levels in the field and greenhouse. Ash and Si concentrations were compared to previously determined values of shoot mass, transpiration, W, and carbon isotope discrimination (Δ). Ash and Si concentrations were not consistently related to Δ and W across all environments; however, ash concentration was positively correlated with Δ (r=0.69**, df= 22) and negatively correlated with W (r= -0.61**, df =22) in the well-watered field environment. Across all environments and studies, the ranges in the coefficients of variation (CV, %) for clonal means were: W, 4-15; Δ, 1-4; ash concentration, 6-14; Si concentration, 13-30; and Si uptake, 21-33. The generally lower CV for W, Δ, and ash concentration suggest that these traits were more repeatable than Si concentration or uptake. Although a consistent relationship was not observed between Si and W and between ash and W, the correlations of ash and W from the well-watered field environment were encouraging. In view of the low cost for ash analysis, we conclude that further research is needed to evaluate the potential of ash as a criterion in selecting for improved W, particularly during the early phases of a breeding programme when large populations are usually involved. Later selections could be based on the more precise and accurate, but costly, Δ analysis.