Abstract
An aseptic dual culture of grape phylloxera ( Daktulosphaira vitifoliae FITCH) and grape vine ( Vitis spp.) was developed. This method permits continuous observation of phylloxera feeding and the whole plant response on a dynamic basis. The plant/parasite interaction of three testplants ( V. vinifera L., var. Riesling, SO 4 ( V. berlandieri PLANCH. X V. riparia L.) and V. riparia , var. Gloire de Montpellier) are demonstrated by observing post-infectious reactions of the host- and population dynamics of the parasite. Different stages of phylloxera could be observed including nymphs, winged phylloxera (alatae) and sexual male phylloxera. Several potential applications for this aseptic dual culture are demonstrated.
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