Ascorbic and Folic Acids Intervention in P. berghei Induced Oxidative Stress in Mice

Abstract

This research attempts to examine the effects of ascorbic and folic acid intervention on the haematology, antioxidants molecules and enzymes of mice exposed to malaria infection. The study involves three groups of control (non-parasitized-nontreated), parasitized-nontreated (PnT) and parasitized ascorbic and folic acid treated (P+as+faT). Intervention with ascorbic and folic acids commenced for three days after parasitemia had been established in mice. Results from this study showed that ascorbic and folic acid intervention in malaria condition reduced (P<0.05) total protein, erythrocyte fragility (EF), increased (P<0.05) packed cell volume (PCV) in comparison with PnT and control mice groups. Lipid peroxidation product in serum, Superoxide dismutase (SOD) activity and Catalase (CAT) activity and reduced glutathione (GSH) reduced in parasitized mice administered with ascorbic and folic acid doses, as against those of control, whereas SOD activity in Control and CAT activity in PnT observed to increase and decrease, respectively. The extent of lipid peroxidation in kidney was effectively reduced by ascorbic and folic acid compared to PnT. In liver SOD activity, CAT activity, glucose-6-phosphate dehydrogenase (G6PD) activity significantly (P<0.05) reduced in P+as+faT as against PnT and control groups. From these observations therefore, we draw the conclusion that ascorbic and folic acids combination in malaria infection may reduce lipid peroxidation and stimulate cellular pathways that enhance the production of high concentrations of hydrogen peroxide.