ASCORBIC ACID

Abstract

Objectives: To demonstrate the volume changes that are produced byanthracycline Doxorubicin DOX on the testes of mice and designed to study volumetric changesin DOX affected testes with co-administration of antioxidant Vitamin C. Design: An experimentalstudy. Setting: Institute of Basic Medical Sciences. Dow University of Health Sciences. Period:September 2011 to May 2012. Methods: Experimental study using thirty male mice of fiveweeks old were separated into 3 groups (A, B & C). Each group had ten mice and was treatedwith normal saline 1 ml intraperitoneal (IP) in group A, DOX alone (0.003 mg in 0.03ml /gmbody weight IP for 3 doses on 6th, 8th and 10th day of study) in group B and DOX (0.003 mgin 0.03ml /gm body weight IP for 3 doses on 6th, 8th and 10th day of study) + Vitamin C (0.5mg in 0.01ml/gm body weight per orumdaily) in group C. After completion of study, animalswere sacrificed and the testes were kept in Bouin’s fluid and volume was measured. Results:By using SPSSS version 16, statistical analysis was done, using ANOVA test to evaluate thesignificance of concerned parameter among different groups studied. The administration ofDOX induced significant reduction (P <0.001) of volume of testes when compared to controls.However co-administration of Vitamin C with DOX significantly increase (P <0.001) the volumeas compared to DOX group. Conclusion: This study suggested that the antioxidant VitaminC has a significant role in ameliorating the damage of testes induced by DOX, showingimprovement in the morphology and morphometry of testes.