ASCORBIC ACID FOLIIAR SPRAY COUNTERACTING EFFECT OF SALINITY ON GROWTH, NUTRIENTS CONCENTRATIONS, PHOTOSYNTHESIS, ANTIOXIDANT ACTIVITIES AND LIPID PEROXIDATION OF BEAN (&lt;i&gt;PHASEULUS VULGARIS&lt;/i&gt; L.) CULTIVARS

Salama Salama

doi:10.3844/ajabssp.2014.384.393

Abstract

A water culture experiment was carried out in the Department of Fertilization Technology at National Research Centre, Cairo, Egypt, to investigate the effect of Ascorbic Acid (AsA) foliar application and salinity stress (100 ppm and 100 mmoL NaCl) on growth, nutrients concentration and some biochemical parameters of two kidney bean (Phaseulus Vulgaris L.) cultivars. Salinity caused significant reduction in growth parameters (leaves and root dry weights) and some of biochemical parameters (nutrients concentration, photosynthesis pigment, Carbonic Anhydrase Activity (CAA), antioxidant enzyme activity Peroxidase (POD) and lipid peroxidation). The reduction effect on Paulista cultivar was higher than Nebraska cultivar at 100 mM NaCl salinity stress. Meanwhile, POD activity was increased under salt stress conditions. Lipid Peroxidation (LP) under 100 mmoL NaCl salinity was significantly increased. The two cultivars showed an increase in MDA content with NaCl salinity stress, but the increase in sensitive cultivars Paulista was higher than that in salt-tolerant Nebraska cultivar. Application of (AsA) not only mitigated the inhibitory effect of salt stress in both kidney bean cultivars, but also in some cases induced a stimulatory effect greater than that estimated in the control plants on growth parameters which were accompanied by marked increases in nutrients concentration and photosynthetic system (pigments and carbonic anhydrase activity). Ascorbic Acid (AsA) has been shown to be an essential antioxidant; agent may act as a scavenger of ROS for mitigating the injury on bio-membranes under salt stress. Therefore, this study suggested that (AsA) application may induce an adaptive response in kidney bean through stimulation of the antioxidant enzymes activities, photosynthesis processes and lower lipid peroxidation, in Nebraska relative to Paulista may contribute to salt tolerance mechanism in Nebraska.

Highlights

Oxygen Species (ROS) are generated in response to stress condition (Dat et al, 2000)
This study suggested that (AsA) application may induce an adaptive response in kidney bean through stimulation of the antioxidant enzymes activities, photosynthesis processes and lower lipid peroxidation, in Nebraska relative to Paulista may contribute to salt tolerance mechanism in Nebraska
Effects of ascorbic acid in mitigating the adverse effects of salt stress have been described to activation of some of the enzymatic reactions

Summary

Introduction

Oxygen Species (ROS) are generated in response to stress condition (Dat et al, 2000). Salinity is one of the most important abiotic stresses physiological and biochemical changes in plants like which affect many aspects of plants metabolism and reduced leaf size, stem extension, root proliferation, reduced reduce growth and crop production (Zhu, 2002). When water use efficiency (Farooq et al, 2009) Alteration in plants are subjected to environmental stresses, Reactive metabolic activities (El-Fouly and Salama, 1999), inhibition. Salama et al / American Journal of Agricultural and Biological Sciences 9 (3): 384-393, 2014 of enzymatic activities (Frova et al, 1999) ionic imbalance and disturbances in solute accumulation (El-Fouly et al, 2010) or a combination of all these factors. Excess of ROS causes phytotoxic reactions such as lipid peroxidation, protein degradation and DNA mutation (Wang et al, 2003)

Methods

Results

Discussion

Conclusion