Abstract
Abstract EPR investigations of spruce needles under in vivo conditions are capable of the detection of several radicals attributed to the plant photosystem . Furthermore, at room temperature an absorption due to ascorbic acid radical and other paramagnetic oxidation products can be detected additionally in the dark-adapted needles as well as under illumination. A systematic investigation of the ascorbic acid signal intensity in spruce needles grown in the vicinity of air quality control units indicates two different oxidative mechanisms, depending on the state of illumination. Ozone plays a key role but is not the hazardous agent monitored by the ascorbic radical immediately. However, its signal reflects the present state of the antioxidative system in the needles and provides an early and simple diagnosis of the oxidative interactions of air pollutants with the photosynthetic apparatus of big trees.
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