Abstract
HeLa cells in culture do not accumulate ascorbic acid unless ascorbic acid or dehydroascorbic acid is available in the medium. Collagen peptidase corresponding to the activity found in the invasive zone of tumours, and acid phosphatase, in HeLa cells cultured under normal conditions, are unaffected by ascorbic acid, but are reduced in cells deprived of carbohydrate. These reduced collagen-peptidase levels, but not acid phosphatase, are restored to the values of normal HeLa cells by ascorbic acid. The relevance of these findings is considered in the context of tumour growth and spread.
Highlights
Summary.-HeLa cells in culture do not accumulate ascorbic acid unless ascorbic acid or dehydroascorbic acid is available in the medium
In tissues exposed to carcinogenic agents (Kennaway et al, 1944; Boyland and Grover, 1961) in proliferating malignant tumours (Musulin et al, 1936) and in human skin secondary tumours derived from lung cancer (Kakar and Wilson, 1974) ascorbic acid (AA) levels are higher than in corresponding normal or surrounding normal tissues, and these findings have implications for tumour growth, invasiveness and metastasis
AA and dehydroascorbic acid (DHAA) were withheld from cultures until 4 h before cell harvesting, when 4 successive portions of the acids were added to the growth media in amounts equivalent hto 11 4 x 10-5M (20 ug/ml) medium at hourly intervals followed by incubation for 1 h
Summary
Summary.-HeLa cells in culture do not accumulate ascorbic acid unless ascorbic acid or dehydroascorbic acid is available in the medium. AA and DHAA were withheld from cultures until 4 h before cell harvesting, when 4 successive portions of the acids were added to the growth media in amounts equivalent hto 11 4 x 10-5M (20 ug/ml) medium at hourly intervals followed by incubation for 1 h.
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