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Abstract
Abstract The effects of ascorbate, the interference of the Jaffé reaction was individually studied by polarography, spectrophotometry, and thin-layer chromatography. Ascorbate was incubated in 28.4 mmol dm−3 picrate blank solution in 0.51 mol dm−3 NaOH at 25.00 ± 0.02 °C and the reactivity of test solution containing ascorbate : picrate was investigated. Picramic acid formed in all ascorbate: 28.4 mmol dm−3 picrate test solutions which explained the observed positive interference of ascorbic acid. No fluorescent product was formed in ascorbate : picrate test solutions. The reactivity of ascorbate : picrate test solutions confirmed that ascorbate was a powerful reducing agent. 2-Furaldehyde which has been listed as one of the degradation products of ascorbic acid, is not identified under the experimental conditions.
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