Ascorbate promotes emission of mercury vapour from plants

Abstract

ABSTRACTMercury vapour (Hg°) emission from plants contributes to the atmospheric mercury cycle. Although a part of this Hg° emission originates from Hg(II) uptake by the roots, the question how terrestrial plants reduce Hg(II) has not been addressed so far. Young barley plants grown on a hydroponic cultivation containing Hg(II) increased the Hg° emission significantly. Homogenates of barley leaves added to dissolved Hg(II) induced a powerful volatilization at alkaline but not at acidic pH. The same pH dependence and emission kinetic together with the highest reduction capacity was observed for ascorbic acid as compared to other phytoreductants. The electrochemical potentials of the reactions involved suggest an electron transfer from NADPH via GSH and ascorbate to Hg(II). The results support the assumption of a novel mechanism how plants transfer reduction equivalents from the antioxidative defense system via ascorbate to reduce Hg(II) ions, thus counteracting mercury toxicity by volatilizing the metal. This effect appears to be assisted by other light‐dependent processes such as transpiration and ascorbate synthesis.