Abstract
The immunologic activity of somatic and metabolic antigens derived solely from in vitro cultured second- and early third-stage Ascaris suum larvae was studied in mice. Eagle's Minimum Essential Medium and Caenorhabditis briggsae medium 75 were used. The results demonstrate for the first time that metabolic and somatic antigens harvested after 12 days from in vitro cultures were capable of inducing resistance to reinfection. The effect on immunity to A. suum in mice of somatic and metabolic antigens from: (1) second- and third-stage in vitro cultured A. suum larvae; (2) metabolic and somatic antigens from third- and fourth-stage A. suum; (3) C. briggsae metabolic antigens; and (4) desalted Haemonchus contortus third-stage larvae exsheathing fluid are described.
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