Abstract
Herein, we report a novel approach to AS1411-templated formation of fluorescent copper nanomaterials and their application to melamine detection. Fluorescent copper nanomaterials were formed at room temperature by using AS1411 as a template and ascorbic acid as reductant. However, the fluorescence intensity decreased obviously in the presence of melamine. Under the optimized conditions, the quenching fluorescence intensities of copper nanomaterials showed a good linear relationship with the concentration of melamine in the range of 50 μmol/L–120 μmol/L, and the correlation coefficient was 0.9823. In addition, the method was successfully applied in the detection of melamine in milk samples. This method was cost-effective and convenient without any labels or complicated operations. Thus, this work successfully develops the capping AS1411 scaffolds of copper nanomaterials detection of melamine.
Highlights
Melamine, chemically known as 2,4,6-triamino-1,3,5-triazine, is rich in terms of nitrogen and heterocyclic organic chemical
Raw materials are mainly used in the production of melamine formaldehyde resin, and this resin is widely used in wood, plastics, paper, textiles, leather, and other industries
E morphology and size of DNA-Cu NPs were tested by transmission electron microscopy, and the DNA-Cu NPs was distributed evenly with a smaller size of about 5 nm and presented a spherical shape. e DNA-Cu NPs synthesized in this experiment is a crystal with a lattice spacing of 0.2479 nm (Figure 2)
Summary
Chemically known as 2,4,6-triamino-1,3,5-triazine, is rich in terms of nitrogen and heterocyclic organic chemical. Raw materials are mainly used in the production of melamine formaldehyde resin, and this resin is widely used in wood, plastics, paper, textiles, leather, and other industries. Melamine can be used as flame retardants, water reducing agents, formaldehyde cleaning agents, etc. Because melamine molecules contain a large number of nitrogen elements, the regular “Kjeldahl method” cannot eliminate the interference of this kind of “pseudoprotein nitrogen” in the detection of protein content in food or feed, so it is illegal. The nonfood chemicals are added to milk, milk powder, and feed to increase protein content in their products. E limit value of melamine in other foods containing more than 15% milk is 1 mg/kg and 2.5 mg/kg. Enzyme-linked immunosorbent assay [10,11,12] and colloidal immunochromatographic strip [13, 14] have been used for the detection of melamine
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